opti-B1-culture-media-by-human-cell-design-1080

Optiβ1®

EndoC-βH1® and EndoC-βH3® Qualified Culture Medium

ApplicationCatalogSize
Culture of EndoC-βH1® and EndoC-βH3® cellsOB1-100100mL
Order Now

Optiβ1®

EndoC-βH1® and EndoC-βH3® Qualified Culture Medium

ApplicationCatalogSize
Culture of EndoC-βH1® and EndoC-βH3® cellsOB1-100100mL
Order Now
opti-B1-culture-media-by-human-cell-design-1080

Product description

Optiβ1® is a medium that was specifically designed for the culture of EndoC-βH1® and EndoC-βH3® cells. Developed to save time and resources through high standardization, Optiβ1® requires no additional supplements and is chemically defined and validated through rigorous internal quality control qualification.

Product information

TypeCell culture medium
CompatibilityEndoC-βH1® cells, EndoC-βH3® cells
FormLiquid
Quantity100mL
SterilitySterile-filtered
Microbiology testingMycoplasma, bacterial, and fungal testing
Technical validationEndoC-βH cell viability and proliferation

Storage and Use conditions

Shipping conditionsDry ice
Storage conditions-80°C at reception, + 4°C after thawing. Protect from light
Shelf life9 months from date of manufacture
Thawing conditionsOvernight at +4°C
Expiration date after thawing2 weeks

Manufacturing and quality system

Optiβ1® is manufactured, including packaging and quality control, at Human Cell Design facility in compliance with Human Cell Design Quality Management System.
FOR RESEARCH USE ONLY.

Certificate of Analysis

Please contact us to ask for an Optiβ1®’s Certificate of Analysis.

Contact us

List of references

1. Blanchi B, Taurand M, Colace C, Thomaidou S, Audeoud C, Fantuzzi F, et al. EndoC-βH5 cells are storable and ready-to-use human pancreatic beta cells with physiological insulin secretion. Mol Metab. 2023 Oct;76:101772. doi: 10.1016/j.molmet.2023.101772.

2. Curnock AP, Bossi G, Kumaran J, Bawden LJ, Figueiredo R, Tawar R, et al. Cell-targeted PD-1 agonists that mimic PD-L1 are potent T cell inhibitors. JCI Insight [Internet]. 2021 Oct 22 [cited 2023 Nov 22];6(20). https://doi.org/10.1172/jci.insight.152468

3. Szczerbinska I, Tessitore A, Hansson LK, Agrawal A, Ragel Lopez A, Helenius M, et al. Large-Scale Functional Genomics Screen to Identify Modulators of Human β-Cell Insulin Secretion. Biomedicines. 2022 Jan 4;10(1):103. doi: 10.3390/biomedicines10010103

4. Baron M, Maillet J, Huyvaert M, Dechaume A, Boutry R, Loiselle H, et al. Loss-of-function mutations in MRAP2 are pathogenic in hyperphagic obesity with hyperglycemia and hypertension. Nat Med. 2019 Nov;25(11):1733–8. https://doi.org/10.1038/s41591-019-0622-0

5. Brawerman G, Pipella J, Thompson PJ. DNA damage to β cells in culture recapitulates features of senescent β cells that accumulate in type 1 diabetes. Mol Metab. 2022 Aug;62:101524. doi: 10.1016/j.molmet.2022.101524