OptiβMax-BF®
EndoC-βH1® optimized medium: Amplify Faster, Function Better, Discover More
| Application | Catalog | Size |
|---|---|---|
| Culture of EndoC-βH1® cells | OBM-100-NO_BSA | 100mL |
OptiβMax-BF®
EndoC-βH1® optimized medium: Amplify Faster, Function Better, Discover More
| Application | Catalog | Size |
|---|---|---|
| Culture of EndoC-βH1® cells | OBM-100-NO_BSA | 100mL |
Product description
OptiβMax-BF® is a medium designed for EndoC-βH1® cell culture. OptiβMax-BF® medium is the BSA-free version of the original version of OptiβMax® medium. OptiβMax-BF® requires to be completed with 2% (w/v) of Fatty acid free fraction V bovine serum albumin (Fatty acid free BSA). For OptiβMax-BF® medium reconstitution, please rigorously respect Fatty acid free BSA reference 10775835001 from Roche to obtain robust and validated results. OptiβMax-BF® enables significant improvement of cell growth and ensures stability and viability of EndoC-βH1® cells from early passages and throughout extended cultures. Compared to published medium, OptiβMax-BF® significantly raises the weekly proliferation index from 1.6 to 2.6, allowing you to optimize each of your experimental designs. OptiβMax-BF® is chemically defined and validated through rigorous internal quality controls.
Time and resources saving
OptiβMax-BF® medium may provide you with a new solution for saving weeks of your lab ressources.
Timeline to get 100 000 000 cells: 6 weeks for OptiβMax® medium versus 11 weeks for Published initial media (1).
1 – Ravassard P, Hazhouz Y, Pechberty S, Bricout-Neveu E, Armanet M, Czernichow P, et al. A genetically engineered human pancreatic ß cell line exhibiting glucose-inducible insulin secretion. J Clin Invest. sept 2011;121(9):3589-97.
Reproducible & robust data
OptiβMax-BF® proposes incomparable EndoC-βH1® cells viability and improved robust cell function over passages.
Average viability of 98% in EndoC-βH1® cells maintained in OptiβMax® medium for 36 passages.
Product information
| Type | Cell culture medium |
| Compatibility | EndoC-βH1® cells |
| Form | Liquid |
| Quantity | 100mL |
| Sterility | Sterile-filtered |
| Microbiology testing | Mycoplasma, bacterial, and fungal testing |
| Technical validation | Viability and proliferation validation of EndoC-βH1® cells |
Storage and Use conditions
| Shipping conditions | Dry ice |
| Storage conditions | -80°C at reception, + 4°C after thawing. Protect from light |
| Use condition | Add 2% (w/v) bovine serum albumin (fatty acid free, fraction V) before use. Recommended reference of bovine serum albumin: Roche, 10775835001. |
| Shelf life | 9 months from date of manufacture |
| Thawing conditions | Overnight at +4°C |
| Expiration date after thawing | 2 weeks |
Manufacturing and quality system
OptiβMax-BF® is manufactured, including packaging and quality control, at Human Cell Design facility in compliance with Human Cell Design Quality Management System.
FOR RESEARCH USE ONLY.
Certificate of Analysis
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