EndoC-βH1®

The human beta cell line of reference

FIRST VALIDATED HUMAN BETA CELL LINE

EndoC-βH1® is a robust and immortalized human beta cell line

This high-quality human pancreatic beta cell line displays a homogeneity that exceeds 99% and an insulin content of 0.5 to 1 µg/million cells. Functionality of EndoC-βH1® cells has been validated using glucose stimulated insulin secretion (GSIS) assay. Our production and assay processes are conducted with rigor and precision, in full respect of the industry’s highest quality standards for reliability and reproducibility. Below are the technical data of EndoC-βH1® characteristics.

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HUMAN BETA CELLS IN UNLIMITED QUANTITIES

EndoC-βH1® is an amplifiable human pancreatic beta cell, while maintaining a significant level of function

Glucose stimulated insulin secretion

Insulin content about 10% that of native human cells

Sensitivity (> 2 fold increased insulin secretion upon high glucose)

Resolution (dozens of ng insulin secreted per million cells)

HTS compatible

Homogeneous cell line > 99%

Robust over 100 passages

Can be genetically engineered for assay development

ALREADY TESTED AND APPROVED BY MORE THAN

Academic & pharmaceutical laboratories worldwide

KEY BENEFITS

Unlimited supply

Compatible with High-Throughput Screening (HTS)

Compatible with genome editing and gene expression modulation strategies (CRISPR, lentiviral shRNA, siRNA…)

Genetic stability over 100 passages

Functional activity (glucose stimulated insulin secretion)

APPLICATIONS

Screening

Target identification and validation

Biological pathway studies and fundamental research

3D models

Type I and II diabetes cell assay development (lipotoxicity, beta cell death, ER stress…)

Beta cell function studies and beta cell physiology

In vivo transplantation

TECHNOLOGY LIMITS

Implementation in your facility required

Relatively low resolution of insulin secretion

Absence of GLP1R mediated response (Exendin-4)

Insulin content about 10% that of native human beta cells

Proliferative whereas native beta cells are quiescent

Very slow amplification rate

DOWNLOADS

Technical validation user guide

QUALITY ASSURANCE

Deposited cell line (Pasteur Institute CNCM)

Short Tandem Repeat analysis available

Functional validation (glucose and incretin)

Batch Release Certificates available

Negative for mycoplasma

FUNCTIONAL VALIDATION

Insulin content

EndoC-βH1® cell insulin content is about 10% that of native human beta cells (close to 1 μg insulin/million cells)

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Glucose stimulated insulin secretion (GSIS)

EndoC-βH1® cells secrete insulin upon glucose stimulation and secretion is further potentiated by IBMX

In Vivo Validation

EndoC-βH1® cells restore normo-glycemia when transplanted into diabetic (STZ) SCID mice

EndoC-βH1®

The human beta cell line of reference

Features & Benefits

Downloads

Functional Validation

Cell Culture Tutorial

Related Products & Services

FIRST VALIDATED HUMAN BETA CELL LINE

EndoC-βH1® is a robust and immortalized human beta cell line

HUMAN BETA CELLS IN UNLIMITED QUANTITIES

EndoC-βH1® is an amplifiable human pancreatic beta cell, while maintaining a significant level of function