Ulti-ST®
EndoC-βH5®, EndoC-βH3® and EndoC-βH1® Qualified Glucose Starvation Medium Dedicated to Glucose-Stimulated Insulin Secretion (GSIS) Assay
| Application | Catalog | Size |
|---|---|---|
| Glucose-stimulated insulin secretion (GSIS) assay on EndoC-βH5®, EndoC-βH3® and EndoC-βH1® cells | UST-50-BSA | 50mL |
U.S. Customers : Due to updated regulations on animal origin products, the import of such items now requires a USDA import permit. To obtain our products and avoid you any supply shortage, we can offer you two options:
2. Opt for our BSA-free medium version, a convenient solution saving you time and resources. Please note that this medium requires the addition of BSA before use.
Ulti-ST®
EndoC-βH5®, EndoC-βH3® and EndoC-βH1® Qualified Glucose Starvation Medium Dedicated to Glucose-Stimulated Insulin Secretion (GSIS) Assay
| Application | Catalog | Size |
|---|---|---|
| Glucose-stimulated insulin secretion (GSIS) assay on EndoC-βH5®, EndoC-βH3® and EndoC-βH1® cells | UST-50-BSA | 50mL |
U.S. Customers : Due to updated regulations on animal origin products, the import of such items now requires a USDA import permit. To obtain our products and avoid you any supply shortage, we can offer you two options:
2. Opt for our BSA-free medium version, a convenient solution saving you time and resources. Please note that this medium requires the addition of BSA before use.
Product description
Ulti-ST® medium was specifically designed for the glucose starvation of EndoC-βH5®, EndoC-βH3® and EndoC-βH1® cells and the maintenance of their high functional activity prior to glucose-stimulated insulin secretion (GSIS) assays. Ulti-ST® requires no additional supplements and is chemically defined and validated through rigorous internal quality control.
Product information
| Type | Glucose starvation medium |
| Compatibility | EndoC-βH5®, EndoC-βH3®, EndoC-βH1® cells |
| Form | Liquid |
| Quantity | 50mL |
| Sterility | Sterile-filtered |
| Microbiology testing | Bacterial and fungal testing |
| Technical validation | Glucose-stimulated insulin secretion |
Storage and Use conditions
| Shipping conditions | Dry ice |
| Storage conditions | -80°C at reception, + 4°C after thawing. Protect from light |
| Shelf life | 9 months from date of manufacture |
| Thawing conditions | Overnight at +4°C |
| Expiration date after thawing | 2 weeks |
Manufacturing and quality system
Ulti-ST® is manufactured, including packaging and quality control, at Human Cell Design facility in compliance with Human Cell Design Quality Management System.
FOR RESEARCH USE ONLY.
Certificate of Analysis
Please contact us to ask for an Ulti-ST®’s Certificate of Analysis.
List of references
1. Blanchi B, Taurand M, Colace C, Thomaidou S, Audeoud C, Fantuzzi F, Sawatani T, Gheibi S, Sabadell-Basallote J, Boot FWJ, Chantier T, Piet A, Cavanihac C, Pilette M, Balguerie A, Olleik H, Carlotti F, Ejarque M, Fex M, Mulder H, Cnop M, Eizirik DL, Jouannot O, Gaffuri AL, Czernichow P, Zaldumbide A, Scharfmann R, Ravassard P.Mol Metab. 2023 Oct;76:101772. doi: 10.1016/j.molmet.2023.101772. Epub 2023 Jul 11.PMID: 37442376. Free PMC article. EndoC-βH5 cells are storable and ready-to-use human pancreatic beta cells with physiological insulin secretion.
2. Frørup C, Gerwig R, Svane CAS, Mendes Lopes de Melo J, Henriksen K, Fløyel T, Pociot F, Kaur S, Størling J.Front Endocrinol (Lausanne). 2023 Apr 11;14:1128523. doi: 10.3389/fendo.2023.1128523. eCollection 2023.PMID: 37113489. Free PMC article. Characterization of the functional and transcriptomic effects of pro-inflammatory cytokines on human EndoC-βH5 beta cells.
3. Brawerman G, Pipella J, Thompson PJ.Mol Metab. 2022 Aug;62:101524. doi: 10.1016/j.molmet.2022.101524. Epub 2022 Jun 2.PMID: 35660116. Free PMC article. DNA damage to β cells in culture recapitulates features of senescent β cells that accumulate in type 1 diabetes.
4. Szczerbinska I, Tessitore A, Hansson LK, Agrawal A, Ragel Lopez A, Helenius M, Malinowski AR, Gilboa B, Ruby MA, Gupta R, Ämmälä C.Biomedicines. 2022 Jan 4;10(1):103. doi: 10.3390/biomedicines10010103.PMID: 35052782. Free PMC article. Large-Scale Functional Genomics Screen to Identify Modulators of Human β-Cell Insulin Secretion.
5. Urbanczyk M, Jeyagaran A, Zbinden A, Lu C en, Marzi J, Kuhlburger L, et al. Matrix Biol. 2023 Jan 1;115:160–83. Decorin improves human pancreatic β-cell function and regulates ECM expression in vitro.
