Noci-One®
Human Nociceptor Neurons
Noci-One®
Human Nociceptor Neurons
Human Cell Design scientists took an original approach to create unique Human Nociceptor Neurons that positively respond to a reference anti-pain molecule Lidocaine in a physiologically relevant functional assay.
READY TO USE HUMAN NOCICEPTOR NEURONS IN UNLIMITED QUANTITIES
Noci-One® neurons are high-quality functional human nociceptor neurons that respond to Lidocaine in a neurotransmitter release assay
NEED TO GO ONE STEP FURTHER?
Human Cell Design team of experts develops assays and provides you with tailored Noci-One® cell models.
KEY BENEFITS
APPLICATIONS
TECHNOLOGY LIMITS
DOWNLOADS
QUALITY ASSURANCE
Batch Release Certificates available
Every batch negatively tested for mycoplasma
Every batch functionally validated
FUNCTIONAL VALIDATION
Phenotypic Characterisation
Noci-One® neurons are an enriched population of TrkA expressing human nociceptor neurons. Flow cytometry analysis shows that a majority of Tuj1+ Noci-One® neurons co-express TrkA.
Morphology
Noci-One® nociceptor neurons show significant TrkA expression in neuron cell bodies. Immunofluorescence analysis showing co-expression of TrkA and Tuj1 in hiPSC derived Noci-One® nociceptor neurons. Typical nociceptor morphology with large round cell bodies and TrkA expression.
Functional Characterisation
Noci-One® human nociceptor neurons respond to reference anti-pain drug Lidocaine in a neurotransmitter release functional assay. Complete inhibition of Veratridine (Vera, 3uM) induced Substance P release upon treatment with reference anti-pain molecule Lidocaine (Lido, 100uM).
