Succeed

Cytokine-Mediated

Human Beta Cell Inflammation Assay

Features & Benefits

Experience high-quality assay tailored to your success

Assay Concept

Discover our assay

Applications & Readouts

Match your project with our assay

Downloads

Find out more about the assay

REVEALING YOUR DRUG POTENTIAL USING A HUMAN PATHOLOGICALLY RELEVANT TYPE 1 AND TYPE 2 DIABETES-MEDIATED INFLAMMATION ASSAY

Evaluate the anti-inflammatory and beta cell protection potential of your drug compounds

Inflammation is a major and complex component of pathogenic processes in type 1 and type 2 diabetes mellitus. In type 1 diabetes, inflammation is a primary event that persists and intensifies as the disease progresses, whereas in type 2 diabetes, inflammation is a chronic feature. In both types of diabetes, the phenomenon involves inflammatory cytokines and is responsible for impaired insulin secretion and the destruction of pancreatic beta cells, making it a central strategy for drug discovery activities targeting Diabetes. Using Endoc-βH5® cells, this assay reproduces the clinically observed response of human pancreatic beta cells to cytokine-induced inflammation. It enables you to assess the pharmacological efficacy of your drug discovery strategies aiming to modulate inflammatory response and protect beta cells from inflammation.

FEATURES & BENEFITS

A unique humanized and pharmacologically validated assay

Recapitulates in vitro the cytokine-mediated inflammation process of type 2 and type 1 diabetes

Human Assay
Pathophysiologically relevant
Screen and profile your compounds at all stages from early inflammation-induced cell dysfunction to apoptosis
Acute and chronic treatment compatible (>3 weeks)
Assess cell protection
Combine functional readout with death and inflammation readouts
HTS compatible

ASSAY CONCEPT

APPLICATIONS

Anti-inflammatory potential
Beta cell protection
Screening campaigns
Specific target validation
Mechanism of action description

READOUTS

Cell function decrease (Insulin secretion)
Cell viability & death
Cell inflammation profile
Beta cell identity markers
Functional markers
Mechanistic markers
Gene expression profile

HAVE A PROJECT IN MIND?

Human Cell Design team of experts can offer you tailored assays and EndoC-βH5® derived cell models.