HLA-A2 EndoC-βH5®

Human Autoimmune Type 1 Diabetes in a Dish

HLA-A2

EndoC-βH5®

Human Autoimmune Type 1 Diabetes in a Dish

REVOLUTIONIZING AUTOIMMUNE TYPE 1 DIABETES DRUG DISCOVERY

Recapitulate beta cell specific attack after T-cell activation and unlock your innovations with a highly pathologically relevant human Type I Diabetes model

T-cell-mediated autoimmunity leading to beta cell death is a key factor in the progression of Type I diabetes. HLA-A2 EndoC-βH5® is a unique human cell model that robustly recapitulates the pathophysiology of T-cell mediated autoimmunity related to Human Type 1 diabetes. The histocompatibility antigen HLA-A*02 is a critical marker present on pancreatic beta cells of a large number of type 1 diabetic patients. HLA-A2 EndoC-βH5® is a cellular model expressing HLA-A*02 that can replicate in vitro the recognition and destruction of beta cells by CD8+ cytotoxic T cells. HLA-A2 Endoc-βH5® offers a unique solution to combine T-cell killing attack with measuring the indirect impact on insulin secretion and function loss.

FEATURES AND BENEFITS

A Pathophysiological Cell Model Recapitulating Autoimmune Human Beta Cell Attack

HLA-A2 EndoC-βH5® cells enable more accurate clinical predictions by modelling the function decrease and beta cell death in response to T-cell attack observed in Human Type 1 Diabetes

T-Cell clone activation assay
HTS compatible
Reproducibility: robust CD8 T-cell mediated beta cell killing
T1D relevance : HLA-A2 positive
Beta cell death impact on insulin secretion
GLP-1/GIP analogs positive response
Flexibility: storable and ready-to-use
Immediately available
Consistent model from first step to preclinical use
Batch-to-batch reproducibility
3D model

A Patho-physiological Cell Model Recapitulating Autoimmune Human Beta Cell Attack

HLA-A2 EndoC-βH5® cells enable more accurate clinical predictions by modelling the function decrease and beta cell death in response to T-cell attack observed in Human Type 1 Diabetes

T1D relevance : HLA-A2 positive
Reproducibility: robust CD8 T-cell mediated beta cell killing
HTS compatible
T-Cell clone activation assay
Beta cell death impact on insulin secretion
GLP-1/GIP analogs positive response
Flexibility: storable and ready-to-use
Immediately available
Consistent model from first step to preclinical use
Batch-to-batch reproducibility
3D model

QUALITY ASSURANCE

Validated
HLA-A*02
expression
Functional validation (glucose and incretin)
Negative for mycoplasma

APPLICATIONS

Beta cell protection
T-cell inactivation
Specific target validation
Mechanism of action description

READOUTS

Beta cell function (Insulin secretion)
Beta cell viability & loss
T-cell killing
T-cell activation (IFN gamma, MIPB1 secretion)

CELL MODEL RELEVANCE

T-cell recognition of HLA-A2 EndoC-BH5

NEED TO GO ONE STEP FURTHER?

Human Cell Design team of experts can offer you tailored assays and HLA-A2 EndoC-βH5® derived cell models.