EndoC-βH1®
The human beta cell line of reference
FIRST VALIDATED HUMAN BETA CELL LINE
EndoC-βH1® is a robust and immortalized human beta cell line
This high-quality human pancreatic beta cell line displays a homogeneity that exceeds 99% and an insulin content of 0.5 to 1 µg/million cells. Functionality of EndoC-βH1® cells has been validated using glucose stimulated insulin secretion (GSIS) assay. Our production and assay processes are conducted with rigor and precision, in full respect of the industry’s highest quality standards for reliability and reproducibility. Below are the technical data of EndoC-βH1® characteristics.
HUMAN BETA CELLS IN UNLIMITED QUANTITIES
EndoC-βH1® is an amplifiable human pancreatic beta cell, while maintaining a significant level of function
Glucose stimulated insulin secretion
Insulin content about 10% that of native human cells
Sensitivity (> 2 fold increased insulin secretion upon high glucose)
Resolution (dozens of ng insulin secreted per million cells)
HTS compatible
Homogeneous cell line > 99%
Robust over 100 passages
Can be genetically engineered for assay development
TESTED AND APPROVED BY MORE THAN 200 ACADEMIC & PHARMACEUTICAL LABORATORIES WORLDWIDE
KEY BENEFITS
Unlimited supply
Compatible with High-Throughput Screening (HTS)
Compatible with genome editing and gene expression modulation strategies (CRISPR, lentiviral shRNA, siRNA…)
Genetic stability over 100 passages
Functional activity (glucose stimulated insulin secretion)
APPLICATIONS
Screening
Target identification and validation
Biological pathway studies and fundamental research
3D models
Type I and II diabetes cell assay development (lipotoxicity, beta cell death, ER stress…)
Beta cell function studies and beta cell physiology
In vivo transplantation
TECHNOLOGY LIMITS
Implementation in your facility required
Relatively low resolution of insulin secretion
Absence of GLP1R mediated response (Exendin-4)
Insulin content about 10% that of native human beta cells
Proliferative whereas native beta cells are quiescent
Very slow amplification rate
DOWNLOADS
QUALITY ASSURANCE
Deposited cell line (Pasteur Institute CNCM)
Short Tandem Repeat analysis available
Batch Release Certificates available
Every batch negatively tested for mycoplasma
Every batch validated for thawing / amplification / GSIS
FUNCTIONAL VALIDATION
Insulin content
EndoC-βH1® cell insulin content is about 10% that of native human beta cells (close to 1 μg insulin/million cells)
Glucose stimulated insulin secretion (GSIS)
EndoC-βH1® cells secrete insulin upon glucose stimulation and secretion is further potentiated by IBMX
In Vivo Validation
EndoC-βH1® cells restore normo-glycemia when transplanted into diabetic (STZ) SCID mice
INDEPENDENT PUBLICATIONS
How other scientists use EndoC-βH1® cells
Kristinsson H, Sargsyan E, Manell H, Smith DM, Göpel SO, Bergsten P. Sci Rep.
Basal hypersecretion of glucagon and insulin from palmitate-exposed human islets depends on FFAR1 but not decreased somatostatin secretion.
2017 Jul 5;7(1):4657.
Basal hypersecretion of glucagon and insulin from palmitate-exposed human islets depends on FFAR1 but not decreased somatostatin secretion.
2017 Jul 5;7(1):4657.
Juan-Mateu J, Rech TH, Villate O, Lizarraga-Mollinedo E, Wendt A, Turatsinze JV, Brondani LA, Nardelli TR, Nogueira TC, Esguerra JL, Alvelos MI, Marchetti P, Eliasson L, Eizirik DL. J Biol Chem.
Neuron-enriched RNA-binding Proteins Regulate Pancreatic Beta Cell Function and Survival.
2017 Feb 24;292(8):3466-3480.
Neuron-enriched RNA-binding Proteins Regulate Pancreatic Beta Cell Function and Survival.
2017 Feb 24;292(8):3466-3480.
Ndiaye FK, Ortalli A, Canouil M, Huyvaert M, Salazar-Cardozo C, Lecoeur C, Verbanck M, Pawlowski V, Boutry R, Durand E, Rabearivelo I, Sand O, Marselli L, Kerr-Conte J, Chandra V, Scharfmann R, Poulain-Godefroy O, Marchetti P, Pattou F, Abderrahmani A, Froguel P, Bonnefond A. Mol Metab.
Expression and functional assessment of candidate type 2 diabetes susceptibility genes identify four new genes contributing to human insulin secretion.
2017 Apr 8;6(6):459-470.
Expression and functional assessment of candidate type 2 diabetes susceptibility genes identify four new genes contributing to human insulin secretion.
2017 Apr 8;6(6):459-470.
Krizhanovskii C, Fred RG, Oskarsson ME, Westermark GT, Welsh N. Ups J Med Sci.
Addition of exogenous sodium palmitate increases the IAPP/insulin mRNA ratio via GPR40 in human EndoC-βH1 cells.
2017 Aug;122(3):149-159.
Addition of exogenous sodium palmitate increases the IAPP/insulin mRNA ratio via GPR40 in human EndoC-βH1 cells.
2017 Aug;122(3):149-159.
Lombardi A, Tomer Y. J Autoimmun.
Interferon alpha impairs insulin production in human beta cells via endoplasmic reticulum stress.
2017 Jun;80:48-55.
Interferon alpha impairs insulin production in human beta cells via endoplasmic reticulum stress.
2017 Jun;80:48-55.
