EndoC-βH1®

The human beta cell line of reference

FIRST VALIDATED HUMAN BETA CELL LINE

EndoC-βH1® is a robust and immortalized human beta cell line

This high-quality human pancreatic beta cell line displays a homogeneity that exceeds 99% and an insulin content of 0.5 to 1 µg/million cells. Functionality of EndoC-βH1® cells has been validated using glucose stimulated insulin secretion (GSIS) assay. Our production and assay processes are conducted with rigor and precision, in full respect of the industry’s highest quality standards for reliability and reproducibility. Below are the technical data of EndoC-βH1® characteristics.

HUMAN BETA CELLS IN UNLIMITED QUANTITIES

EndoC-βH1® is an amplifiable human pancreatic beta cell, while maintaining a significant level of function

Glucose stimulated insulin secretion
Insulin content about 10% that of native human cells
Sensitivity (> 2 fold increased insulin secretion upon high glucose)
Resolution (dozens of ng insulin secreted per million cells)
HTS compatible
Homogeneous cell line > 99%
Robust over 100 passages
Can be genetically engineered for assay development

ALREADY TESTED AND APPROVED BY MORE THAN

Academic & pharmaceutical laboratories worldwide

HCD Users Worldwide

KEY BENEFITS

Unlimited supply
Compatible with High-Throughput Screening (HTS)
Compatible with genome editing and gene expression modulation strategies (CRISPR, lentiviral shRNA, siRNA…)
Genetic stability over 100 passages
Functional activity (glucose stimulated insulin secretion)

APPLICATIONS

Screening
Target identification and validation
Biological pathway studies and fundamental research
3D models
Type I and II diabetes cell assay development (lipotoxicity, beta cell death, ER stress…)
Beta cell function studies and beta cell physiology
In vivo transplantation

TECHNOLOGY LIMITS

Implementation in your facility required
Relatively low resolution of insulin secretion
Absence of GLP1R mediated response (Exendin-4)
Insulin content about 10% that of native human beta cells
Proliferative whereas native beta cells are quiescent
Very slow amplification rate

QUALITY ASSURANCE

Deposited cell line (Pasteur Institute CNCM)
Short Tandem Repeat analysis available
Functional validation (glucose and incretin)
Batch Release Certificates available
Negative for mycoplasma

FUNCTIONAL VALIDATION

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Insulin content

EndoC-βH1® cell insulin content is about 10% that of native human beta cells (close to 1 μg insulin/million cells)

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Glucose stimulated insulin secretion (GSIS)

EndoC-βH1® cells secrete insulin upon glucose stimulation and secretion is further potentiated by IBMX

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In Vivo Validation

EndoC-βH1® cells restore normo-glycemia when transplanted into diabetic (STZ) SCID mice

EndoC-βH1® CELL CULTURE TUTORIAL

Similar cell culture technique as EndoC-βH5® Cells