
EndoC-βH3®
A human beta cell with conditional immortalisation
ONE STEP CLOSER TO NATIVE HUMAN BETA CELLS
EndoC-βH3® human pancreatic beta cell line is an intermediate version of EndoC-βH® cells. EndoC-βH3® cells are conditionally immortalized and de-immortalization leads to an increase in insulin content and secretion. EndoC-βH3® cells thus represent a translational tool to study beta cell proliferation and apoptosis.
FUNCTIONALLY AND CONDITIONNALY IMMORTALIZED HUMAN BETA CELL
EndoC-βH3® is a conditional excisable high-quality functional human beta cell line
Glucose stimulated insulin secretion
Insulin content up to 20% that of native human beta cells
Insulin content about 20% of native human beta cells
Sensibility > 2 fold increase 20mM glucose
HTS compatible
Homogeneous cell line > 99%
Robust over 85 passages
Can be genetically engineered for assay development
ALREADY TESTED AND APPROVED BY MORE THAN
Academic & pharmaceutical laboratories worldwide
KEY BENEFITS
Unlimited supply
Conditionally quiescent
Compatible with High-Throughput Screening (HTS)
Compatible with genome editing and gene expression modulation strategies (CRISPR, lentiviral shRNA, siRNA…)
Genetic stability over 85 passages
APPLICATIONS
Beta cell de-differentiation and maturation
Beta cell apoptosis
Beta cell proliferation
Type I and II diabetes cell assay development
In vivo transplantation
TECHNOLOGY LIMITS
Implementation in your facility required
Lack of GSIS robustness
Lack of response to GLP1 analog Exendin-4
Lack of respone to GIP analog