βKrebs®
EndoC-βH5®, EndoC-βH3® and EndoC-βH1® Qualified Krebs Buffer Solution Dedicated to Glucose-Stimulated Insulin Secretion (GSIS) Assay
Application | Catalog | Size |
---|---|---|
Glucose-stimulated insulin secretion (GSIS) assay on EndoC-βH5®, EndoC-βH3® and EndoC-βH1® cells | BK-100 | 100mL |
βKrebs®
EndoC-βH5®, EndoC-βH3® and EndoC-βH1® Qualified Krebs Buffer Solution Dedicated to Glucose-Stimulated Insulin Secretion (GSIS) Assay
Application | Catalog | Size |
---|---|---|
Glucose-stimulated insulin secretion (GSIS) assay on EndoC-βH5®, EndoC-βH3® and EndoC-βH1® cells | BK-100 | 100mL |
Product description
βKrebs® is a Krebs based buffer specifically designed for Glucose-stimulated insulin secretion (GSIS) assays on EndoC-βH5®, EndoC-βH3® and EndoC-βH1® cells.
Product information
Type | Glucose-stimulated insulin secretion (GSIS) buffer |
Compatibility | EndoC-βH5® cells, EndoC-βH3® cells, EndoC-βH1® cells |
Form | Liquid |
Quantity | 100mL |
Sterility | Sterile-filtered |
Microbiology testing | Bacterial and fungal testing |
Technical validation | Glucose-stimulated insulin secretion |
Storage and Use conditions
Shipping conditions | Room temperature |
Storage conditions | +4°C at reception. Protect from light |
Shelf life | 6 months from date of manufacture |
Manufacturing and quality system
βKrebs® is manufactured, including packaging and quality control, at Human Cell Design facility in compliance with Human Cell Design Quality Management System.
FOR RESEARCH USE ONLY.
Certificate of Analysis
Please contact us to ask for a βKrebs®’s Certificate of Analysis.
List of references
1. Blanchi B, Taurand M, Colace C, Thomaidou S, Audeoud C, Fantuzzi F, Sawatani T, Gheibi S, Sabadell-Basallote J, Boot FWJ, Chantier T, Piet A, Cavanihac C, Pilette M, Balguerie A, Olleik H, Carlotti F, Ejarque M, Fex M, Mulder H, Cnop M, Eizirik DL, Jouannot O, Gaffuri AL, Czernichow P, Zaldumbide A, Scharfmann R, Ravassard P.Mol Metab. 2023 Oct;76:101772. doi: 10.1016/j.molmet.2023.101772. Epub 2023 Jul 11.PMID: 37442376. Free PMC article. EndoC-βH5 cells are storable and ready-to-use human pancreatic beta cells with physiological insulin secretion.
2. Frørup C, Gerwig R, Svane CAS, Mendes Lopes de Melo J, Henriksen K, Fløyel T, Pociot F, Kaur S, Størling J.Front Endocrinol (Lausanne). 2023 Apr 11;14:1128523. doi: 10.3389/fendo.2023.1128523. eCollection 2023.PMID: 37113489. Free PMC article. Characterization of the functional and transcriptomic effects of pro-inflammatory cytokines on human EndoC-βH5 beta cells.
3. Brawerman G, Pipella J, Thompson PJ.Mol Metab. 2022 Aug;62:101524. doi: 10.1016/j.molmet.2022.101524. Epub 2022 Jun 2.PMID: 35660116. Free PMC article. DNA damage to β cells in culture recapitulates features of senescent β cells that accumulate in type 1 diabetes.
4. Szczerbinska I, Tessitore A, Hansson LK, Agrawal A, Ragel Lopez A, Helenius M, Malinowski AR, Gilboa B, Ruby MA, Gupta R, Ämmälä C.Biomedicines. 2022 Jan 4;10(1):103. doi: 10.3390/biomedicines10010103.PMID: 35052782. Free PMC article. Large-Scale Functional Genomics Screen to Identify Modulators of Human β-Cell Insulin Secretion.