BKREBS

βKrebs®

EndoC-βH5®, EndoC-βH3® and EndoC-βH1® Qualified Krebs Buffer Solution Dedicated to Glucose-Stimulated Insulin Secretion (GSIS) Assay

ApplicationCatalogSize
Glucose-stimulated insulin secretion (GSIS) assay on EndoC-βH5®, EndoC-βH3® and EndoC-βH1® cellsBK-100100mL
Order Now

βKrebs®

EndoC-βH5®, EndoC-βH3® and EndoC-βH1® Qualified Krebs Buffer Solution Dedicated to Glucose-Stimulated Insulin Secretion (GSIS) Assay

ApplicationCatalogSize
Glucose-stimulated insulin secretion (GSIS) assay on EndoC-βH5®, EndoC-βH3® and EndoC-βH1® cellsBK-100100mL

Product description

βKrebs® is a Krebs based buffer specifically designed for Glucose-stimulated insulin secretion (GSIS) assays on EndoC-βH5®, EndoC-βH3® and EndoC-βH1® cells.

Product information

TypeGlucose-stimulated insulin secretion (GSIS) buffer
CompatibilityEndoC-βH5® cells, EndoC-βH3® cells, EndoC-βH1® cells
FormLiquid
Quantity100mL
SterilitySterile-filtered
Microbiology testingBacterial and fungal testing
Technical validationGlucose-stimulated insulin secretion

Storage and Use conditions

Shipping conditionsRoom temperature
Storage conditions+4°C at reception. Protect from light
Shelf life6 months from date of manufacture

Manufacturing and quality system

βKrebs® is manufactured, including packaging and quality control, at Human Cell Design facility in compliance with Human Cell Design Quality Management System.
FOR RESEARCH USE ONLY.

Certificate of Analysis

Please contact us to ask for a βKrebs®’s Certificate of Analysis.

Contact us

List of references

1. Blanchi B, Taurand M, Colace C, Thomaidou S, Audeoud C, Fantuzzi F, Sawatani T, Gheibi S, Sabadell-Basallote J, Boot FWJ, Chantier T, Piet A, Cavanihac C, Pilette M, Balguerie A, Olleik H, Carlotti F, Ejarque M, Fex M, Mulder H, Cnop M, Eizirik DL, Jouannot O, Gaffuri AL, Czernichow P, Zaldumbide A, Scharfmann R, Ravassard P.Mol Metab. 2023 Oct;76:101772. doi: 10.1016/j.molmet.2023.101772. Epub 2023 Jul 11.PMID: 37442376. Free PMC article. EndoC-βH5 cells are storable and ready-to-use human pancreatic beta cells with physiological insulin secretion.

2. Frørup C, Gerwig R, Svane CAS, Mendes Lopes de Melo J, Henriksen K, Fløyel T, Pociot F, Kaur S, Størling J.Front Endocrinol (Lausanne). 2023 Apr 11;14:1128523. doi: 10.3389/fendo.2023.1128523. eCollection 2023.PMID: 37113489. Free PMC article. Characterization of the functional and transcriptomic effects of pro-inflammatory cytokines on human EndoC-βH5 beta cells.

3. Brawerman G, Pipella J, Thompson PJ.Mol Metab. 2022 Aug;62:101524. doi: 10.1016/j.molmet.2022.101524. Epub 2022 Jun 2.PMID: 35660116. Free PMC article. DNA damage to β cells in culture recapitulates features of senescent β cells that accumulate in type 1 diabetes.

4. Szczerbinska I, Tessitore A, Hansson LK, Agrawal A, Ragel Lopez A, Helenius M, Malinowski AR, Gilboa B, Ruby MA, Gupta R, Ämmälä C.Biomedicines. 2022 Jan 4;10(1):103. doi: 10.3390/biomedicines10010103.PMID: 35052782. Free PMC article. Large-Scale Functional Genomics Screen to Identify Modulators of Human β-Cell Insulin Secretion.