CM-One®
Human Ventricular Cardiomyocytes
A CARDIOTOXICOLOGY IN VITRO TOOL
The human ventricular cardiomyocytes: CM-One®
Millions have been spent over the years to identify a way to obtain large quantities of functional human cardiomyocytes, with little success so far. Human Cell Design’s founding scientists took an original approach by initially looking for adult ventricular cardiomyocyte markers. They succeeded in producing and selecting mature ventricular cardiomyocytes. CM-One® pure human ventricular cardiomyocytes have then been functionally and pharmacologically validated using electrophysiological methods. CM-One® cardiomyocytes now represent a valuable translational tool for toxicity and efficacy screening studies, as well as for more fundamental research.
READY TO USE HUMAN VENTRICULAR CARDIOMYOCYTES IN UNLIMITED QUANTITIES
CM-One® are high-quality functional human ventricular cardiomyocytes
NEED TO GO ONE STEP FURTHER?
The Human Cell Design team of experts can provide you with a tailored CM-One® cell model using genome editing technologies.
KEY BENEFITS
APPLICATIONS
TECHNOLOGY LIMITS
QUALITY ASSURANCE
Functional validation
Batch Release Certificates available
Negative for mycoplasma
FUNCTIONAL VALIDATION
Phenotypic Characterisation
CM-One® is a pure population of human cardiomyocytes. CM-One® highly enriched cultures of hiPSc derived cardiomyocytes (B; >95% cTnT positive in FACS analysis) showed high level of sarcomeric organization (A; Nkx2.5/αAct: alpha-actinin).
Functional Characterisation
Action potential (AP) fired by CM-One® cardiomyocytes display features of human ventricular cardiomyocyte AP. AP recording from CM-One® cardiomyocytes measured in whole-cell patch clamp experiment (A) and AP parameters (B) showed adult human ventricular cardiomyocyte-like characteristics.
Functional Characterisation
CM-One® cardiomyocytes show impaired contractility and field potentials upon Nifedipine treatment in impedance and MEA recording. Nifedipine treatment reduces contractility and field potential duration of CM-One® cardiomyocytes, as shown by impedance and MEA measurements using the xCELLigence RTCA CardioECR system.