Optiβ3-BF®
ECN 90® Qualified Culture Medium
| Application | Catalog | Size |
|---|---|---|
| Culture of ECN 90® cells | OB3-100-NO_BSA | 100mL |
Optiβ3®
ECN 90® Qualified Culture Medium
| Application | Catalog | Size |
|---|---|---|
| Culture of ECN 90® cells | OB3-100-BSA | 100mL |
Product description
Optiβ3-BF® is a medium specifically designed for the culture of ECN 90® cells. Optiβ3-BF® medium is the BSA-free version of the original version of Optiβ3® medium. Optiβ3-BF® requires to be completed with 2% (w/v) of Fatty acid free fraction V bovine serum albumin (Fatty acid free BSA). For Optiβ3-BF® medium reconstitution, please rigorously respects Fatty acid free BSA reference 10775835001 from Roche to obtain robust and validated results. Optiβ3-BF® is chemically defined and validated through rigorous internal quality control qualification.
Product information
| Type | Cell culture medium |
| Compatibility | ECN 90® cells |
| Form | Liquid |
| Quantity | 100mL |
| Sterility | Sterile-filtered |
| Microbiology testing | Mycoplasma, bacterial, and fungal testing |
| Technical validation | ECN90® viability and proliferation |
Storage and Use conditions
| Shipping conditions | Dry ice |
| Storage conditions | -80°C at reception, + 4°C after thawing. Protect from light |
| Use condition | Add 2% (w/v) bovine serum albumin (fatty acid free, fraction V) before use. Recommended reference of bovine serum albumin: Roche, 10775835001 |
| Shelf life | 9 months from date of manufacture |
| Thawing conditions | Overnight at +4°C |
| Expiration date after thawing | 2 weeks |
Manufacturing and quality system
Optiβ3® is manufactured, including packaging and quality control, at Human Cell Design facility in compliance with Human Cell Design Quality Management System.
FOR RESEARCH USE ONLY.
Certificate of Analysis
Please contact us to ask for an Optiβ3®’s Certificate of Analysis.
List of references
2. Curnock AP, Bossi G, Kumaran J, Bawden LJ, Figueiredo R, Tawar R, et al. Cell-targeted PD-1 agonists that mimic PD-L1 are potent T cell inhibitors. JCI Insight. 2021 Oct 22;6(20). Available from: https://doi.org/10.1172/jci.insight.152468
3. Rachdi, Latif, Alicia Maugein, Severine Pechberty, Mathieu Armanet, Juliette Hamroune, Philippe Ravassard, Stefano Marullo, Olivier Albagli, et Raphael Scharfmann. « Regulated Expression and Function of the GABAB Receptor in Human Pancreatic Beta Cell Line and Islets ». Scientific Reports 10, no 1 (10 Aug 2020). Available from: https://doi.org/10.1038/s41598-020-69758-6.
