βCoat®
EndoC-βH5®, EndoC-βH3®, EndoC-βH1® and ECN90® Cells Qualified Coating Solution
| Application | Catalog | Size |
|---|---|---|
| Culture of EndoC-βH5®, EndoC-βH3® and EndoC-βH1® and ECN90® cells | BC-120 | 90-130µL / vial |
Product description
βCoat® is a coating solution specifically designed for the culture of EndoC-βH5®, EndoC-βH3®, EndoC-βH1® and ECN90® cells.
Application
Each vial of βCoat® contains between 90µL and 130µL of βCoat® reagent, depending on the batch’s concentration, and allows to prepare 10 mL of coating solution.
Product information
| Type | Coating reagent |
| Compatibility | EndoC-βH5® cells, EndoC-βH3® cells, EndoC-βH1® cells |
| Form | Liquid |
| Quantity | 90-130µL/vial (quantity of product may vary depending on the concentration) |
| Sterility | Sterile-filtered |
| Microbiology testing | Bacterial and fungal testing |
| Technical validation | EndoC-βH cell viability and proliferation |
Storage and Use conditions
| Shipping conditions | Dry ice |
| Storage conditions | -80°C at reception. Protect from light |
| Shelf life | 6 months from date of manufacture |
| Expiration date after thawing | Immediate use |
Manufacturing and quality system
βCoat® is manufactured, including packaging and quality control, at Human Cell Design facility in compliance with Human Cell Design Quality Management System.
FOR RESEARCH USE ONLY.
Certificate of Analysis
Please contact us to ask for a βCoat®’s Certificate of Analysis.
List of references
1. Blanchi B, Taurand M, Colace C, Thomaidou S, Audeoud C, Fantuzzi F, Sawatani T, Gheibi S, Sabadell-Basallote J, Boot FWJ, Chantier T, Piet A, Cavanihac C, Pilette M, Balguerie A, Olleik H, Carlotti F, Ejarque M, Fex M, Mulder H, Cnop M, Eizirik DL, Jouannot O, Gaffuri AL, Czernichow P, Zaldumbide A, Scharfmann R, Ravassard P.Mol Metab. 2023 Oct;76:101772. doi: 10.1016/j.molmet.2023.101772. Epub 2023 Jul 11.PMID: 37442376. Free PMC article. EndoC-βH5 cells are storable and ready-to-use human pancreatic beta cells with physiological insulin secretion.
2. Frørup C, Gerwig R, Svane CAS, Mendes Lopes de Melo J, Henriksen K, Fløyel T, Pociot F, Kaur S, Størling J.Front Endocrinol (Lausanne). 2023 Apr 11;14:1128523. doi: 10.3389/fendo.2023.1128523. eCollection 2023.PMID: 37113489. Free PMC article. Characterization of the functional and transcriptomic effects of pro-inflammatory cytokines on human EndoC-βH5 beta cells.
3. Brawerman G, Pipella J, Thompson PJ.Mol Metab. 2022 Aug;62:101524. doi: 10.1016/j.molmet.2022.101524. Epub 2022 Jun 2.PMID: 35660116. Free PMC article. DNA damage to β cells in culture recapitulates features of senescent β cells that accumulate in type 1 diabetes.
