Insulin Secretion Assays
GSIS & cAMP beta cell function assays
FUNCTIONAL AND PHYSIOLOGICAL ASSAYS VALIDATED FOR GLP-1/GIP REFERENCE COMPOUNDS
Two complementary assays using human primary-like pancreatic beta cells
KEY ADVANTAGES
GSIS BETA CELL FUNCTION GOLD STANDARD
HIGH THROUGHPUT COST-EFFECTIVE cAMP
DATA COMPARABILITY BETWEEN GSIS & cAMP
VALIDATED FOR GLP1-R & GIP-R COMPOUNDS
PHYSIOLOGICAL & TRANSLATIONAL NATIVE-LIKE MODEL
Insulin secretagogues and GLP-1 and GIP incretins regulate blood glucose by potentiating pancreatic insulin secretion. They now represent a treatment of reference for patients with type 2 diabetes. Human Cell Design proposes 2 complementary EndoC-βH5®-based assays dedicated to drug screening campaigns:
cAMP accumulation represents a rapid and cost-effective assay for fast & sensitive drug ranking for medium & high throughput screening.
Glucose Stimulated Insulin Secretion (GSIS) potentiation assay represents a direct & physiologically relevant gold standard widely recognized by academic research, for evaluation of your candidate on the beta cell function.
| GSIS | cAMP | |
|---|---|---|
| Readout | Physiological | Physiological |
| Measurement | Direct | Indirect |
| Throughput | 12 - 24 - 48 - 96 wells | 12 - 24 - 48 - 96 - 384 wells |
| Cost | Medium | Low |
| Sensitivity | High | High |
| Resolution | High | High |
APPLICATIONS
High resolution & translational assays for efficient compound ranking
Screen your molecules with validated references GLP1/GIP-RA compounds
ASSAY CONCEPT
RESULTS
Glucose Stimulated Insulin Secretion (GSIS) gold-standard functional assay
EC50 of of GLP1/GIP-receptor agonists based on dose-response of potentiation of GSIS (11mM of glucose)
Physiological assay with GLP1/GIP analogs response
High sensitivity with 4-fold-increase in insulin secretion upon GLP1 reference compounds
ELISA standardized assay with high reproducibility
8 compounds/plate on 96-wells format
Rapid hightroughput & cost-effective cAMP-based assay
95% of GSIS/cAMP correlation on GLP1-R agonists ranking
HTRF standardized assay with high reproducibility
38 compounds/plate on 384-wells format
Cost effective assay for rapid screening campaign
GSIS & cAMP potency assays correlation of pEC50 means for 3 GLP-1R agonists
DOWNLOADS
Find out more about the two assays physiological and pharmacological responses
HAVE A PROJECT IN MIND?
Human Cell Design team of experts can offer you tailored assays and EndoC-βH5® derived cell models.
FIND THE RIGHT SERVICE FOR YOUR STUDY
Human Cell Design provides tailored services specifically designed for accelerating clinically relevant scientific progress in Type 1 and Type 2 diabetes research. From target validation to preclinical validation, our experts provide you with long term strategies and help you develop stables readouts and valuable deliverables.
