Insulin Secretion
GSIS & cAMP beta cell function assays
KEY ADVANTAGES
Two complementary assays using human primary-like pancreatic beta cells
GSIS
GOLD STANDARD
cAMP
COST-EFFECTIVE ASSAY
cAMP
INDIRECT FUNCTIONAL READOUT
EndoC-βH5®
ENDOGENEOUS GLP1/GIPR
EndoC-βH5®
DATA REPRODUCIBILITY
Incretins and insulin secretagogues represent treatments of reference for type 2 diabetes & Obesity. Human Cell Design offers two assays for testing your therapeutic approaches in function of your product development stage:
- cAMP assay: Cost-effective & scalable
- GSIS (Glucose Stimulated Insulin Secretion) assay: Direct & physiological gold standard
| GSIS | cAMP | |
|---|---|---|
| Readout | Physiological | Physiological |
| Measurement | Direct | Indirect |
| Throughput | 12 - 24 - 48 - 96 wells | 12 - 24 - 48 - 96 - 384 wells |
| Cost | Medium | Low |
| Sensitivity | High | High |
| Resolution | High | High |
APPLICATIONS
High resolution & translational assays for efficient compound ranking
Screen your molecules with validated references GLP1/GIP-RA compounds
ASSAY CONCEPT
RESULTS
Glucose Stimulated Insulin Secretion (GSIS) gold-standard functional assay
EC50 of GLP1/GIP-receptor agonists based on dose-response of potentiation of GSIS (11mM of glucose)
GLP1/GIP analogs physiological responses
High sensitivity
ELISA standardized assay
Hightroughput & cost-effective cAMP-based assay
95% of GSIS/cAMP correlation
HTRF standardized assay
384-wells compatible
Cost-effective solution
GSIS & cAMP potency assays correlation of pEC50 means for 3 GLP-1R agonists
DOWNLOADS
Get more technical & validation data by downloading our technical sheet
HAVE A PROJECT IN MIND?
Human Cell Design team of experts can offer you tailored assays and EndoC-βH5® derived cell models.
FIND THE RIGHT SERVICE FOR YOUR STUDY
Human Cell Design provides tailored services specifically designed for accelerating clinically relevant scientific progress in Type 1 and Type 2 diabetes research. From target validation to preclinical validation, our experts provide you with long term strategies and help you develop stables readouts and valuable deliverables.
