T-Cell Killing
Type 1 Diabetes
KEY ADVANTAGES
Model the immune response of type 1 diabetes
HLA-A2+ MODEL
T-CELL DESTRUCTION
INFLAMMATORY CYTOKINE SECRETION
PBMCs & SORTED T-CELLS
Type 1 diabetes is characterized by the autoimmune destruction of pancreatic β-cells, largely driven by pro-inflammatory T cells. Modeling these immune–β-cell interactions is key for understanding disease mechanisms and developing immunomodulatory therapies.
Human Cell Design offers a physiologically relevant co-culture assay based on HLA-A2 EndoC-βH5® human pancreatic β cells and activated T cells, enabling direct assessment of T cell-mediated cytotoxicity.
APPLICATIONS
A human-relevant system to assess β-cell vulnerability to immune attack
Screen your compounds for β-cell protective or immunomodulatory properties using a reproducible, translational assay
ASSAY CONCEPT
RESULTS
HLA-A2 EndoC–βH5® based assay
MIPβ T-cell secretion in coculture with HLA-A2 EndoC-βH5®
2-fold-increase of T-cell activation under IFNγ
Baricitinib JAK1/2 inihibitor T-cell protection
HLA-A2 EndoC-βH5® & HLA-A2 restricted CD8 T Lymphocytes coculture
DOWNLOAD THE ASSAY DATA SHEET
T-cell killing assay: Get more technical data by downloading our technical sheet
HAVE A PROJECT IN MIND?
Human Cell Design team of experts can offer you tailored assays on HLA-A2 EndoC-βH5® derived cell models.
Other HLA class I-expressing models can also be developed on demand to match your specific immunological context.
