Target Validation
Reinvent diabetes
KEY ADVANTAGES
Reinvent Diabetes: Unlock the Full Potential of Your Target
KO/KD COMPATIBLE
DATA ROBUSTNESS
RAPID
PHYSIOLOGICAL READOUTS
Leverage the unique translational relevance of EndoC-βH5® for gene knock-out and knock-down studies using a full range of genome editing technologies.
Evaluate gene impact on beta cell function, survival and metabolism readouts for a clear and rapid target validation.
| Target gene invalidation | Molecular tools | Readouts |
|---|---|---|
| Knock-Down | siRNA | qPCR |
| miRNA | Western Blot | |
| Lentiviral shRNA | Live-cells Cytometry | |
| Immunofluorescence | ||
| Knock-Out | CRISPR |
APPLICATIONS
A versatile toolbox for a powerful informed decision-making on your target gene
Access to a rapid and robust proof of concept
Stable native like human beta cell
Data reproducibility
siRNA/shRNA: >80% of knockdown
miRNA: >99% of knockdown
Direct functional readouts
Multiple readouts panel
ASSAY CONCEPT
RESULTS
Target gene invalidation
qPCR RNA relative expression of 2 targeted gene siRNAs
siRNA selection: >80% gene invalidation
High well-to-well reproducibility
HAVE A PROJECT IN MIND?
Human Cell Design team of experts can offer you tailored assays and EndoC-βH5® derived cell models.
