EndoC-βH3®
A human beta cell with conditional immortalisation
ONE STEP CLOSER TO NATIVE HUMAN BETA CELLS
EndoC-βH3® human pancreatic beta cell line is an intermediate version of EndoC-βH® cells. EndoC-βH3® cells are conditionally immortalized and de-immortalization leads to an increase in insulin content and secretion. EndoC-βH3® cells thus represent a translational tool to study beta cell proliferation and apoptosis.
FUNCTIONALLY AND CONDITIONNALY IMMORTALIZED HUMAN BETA CELL
EndoC-βH3® is a conditional excisable high-quality functional human beta cell line
ALREADY TESTED AND APPROVED BY MORE THAN
Academic & pharmaceutical laboratories worldwide
KEY BENEFITS
APPLICATIONS
TECHNOLOGY LIMITS
DOWNLOADS
QUALITY ASSURANCE
Deposited cell line (Pasteur Institute CNCM)
Short Tandem Repeat analysis available
Batch Release Certificates available
Every batch negatively tested for mycoplasma
Every batch validated for thawing / amplification / GSIS
FUNCTIONAL VALIDATION
Insulin content
Insulin content of de-immortalized EndoC-βH3® cells is 10-20 % that of native human beta cells (~1-2 μg/million cells).
Glucose stimulated insulin secretion (GSIS)
De-immortalized EndoC-βH3® cells secrete insulin upon glucose stimulation; secretion is further potentiated by IBMX.